Rox8 promotes microRNA-dependent yki messenger RNA decay
| Autor: | Xiaolong Yang, Peng Liu, Xianjue Ma, Xiao-Ping Zhang, Jingjing Luo, Meixiao Zhan, Lei Xue, Yingqun Zhou, Shuai Yang, Xiaowei Guo, Ligong Lu, Yihao Sun, Taha Azad, Zhongwei Lv, H. J. Janse van Rensburg |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Untranslated region
endocrine system 0303 health sciences Hippo signaling pathway Messenger RNA animal structures Multidisciplinary fungi Regulator Biology medicine.disease_cause Cell biology body regions 03 medical and health sciences 0302 clinical medicine In vivo 030220 oncology & carcinogenesis microRNA medicine Carcinogenesis 030304 developmental biology Genetic screen |
| Zdroj: | Proceedings of the National Academy of Sciences. 117:30520-30530 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2013449117 |
| Popis: | The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the Drosophila RasV12/lgl−/− in vivo tumor model and identified Rox8, a RNA-binding protein (RBP), as a positive regulator of the Hippo pathway. We found that Rox8 overexpression suppresses whereas Rox8 depletion potentiates Hippo-dependent tissue overgrowth, accompanied by altered Yki protein level and target gene expression. Mechanistically, Rox8 directly binds to a target site located in the yki 3′ UTR, recruits and stabilizes the targeting of miR-8–loaded RISC, which accelerates the decay of yki messenger RNA (mRNA). Moreover, TIAR, the human ortholog of Rox8, is able to promote the degradation of yki mRNA when introduced into Drosophila and destabilizes YAP mRNA in human cells. Thus, our study provides in vivo evidence that the Hippo pathway is posttranscriptionally regulated by the collaborative action of RBP and microRNA (miRNA), which may provide an approach for modulating Hippo pathway-mediated tumorigenesis. |
| Databáze: | OpenAIRE |
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