Fructose-1,6-Bisphosphatase 1 Reduces18F FDG Uptake in Hepatocellular Carcinoma
Autor: | Ruohua Chen, Gang Huang, Jiajin Li, Xiang Zhou, Jianjun Liu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Gastroenterology 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Carcinoma Radiology Nuclear Medicine and imaging Fluorodeoxyglucose medicine.diagnostic_test biology business.industry medicine.disease 030104 developmental biology HIF1A Real-time polymerase chain reaction Positron emission tomography 030220 oncology & carcinogenesis Hepatocellular carcinoma biology.protein Immunohistochemistry GLUT1 Nuclear medicine business medicine.drug |
Zdroj: | Radiology. 284:844-853 |
ISSN: | 1527-1315 0033-8419 |
Popis: | Purpose To determine whether fructose 1,6-bisphosphatase 1 (FBP1) expression is associated with fluorine 18 (18F) fluorodeoxyglucose (FDG) accumulation in patients with hepatocellular carcinoma (HCC) and to investigate how FBP1 expression and 18F FDG uptake are related to tumor differentiation grade and metabolism and whether the molecular mechanism involves hypoxia-inducible factor 1-α (HIF1A) transcriptional activity. Materials and Methods This retrospective study was approved by the institutional review board with informed consent. Eighty-five patients with HCC underwent 18F FDG combined positron emission tomography and computed tomography (PET/CT). The relationship between maximum standardized uptake (SUVmax) and expression of FBP1, glucose transporter 1 (GLUT1), and hexokinase 2 (HK2) was analyzed with immunohistochemical analysis. In vitro FBP1 overexpression in HCC cells was used to examine the role of FBP1 in tumor metabolism, and its effect on HIF1A transcriptional activity was investigated with quantitative polymerase chain reaction and luciferase reporter assay. Spearman rank correlation was applied to determine the association between FBP1 expression and SUVmax. Results There was an inverse relationship between FBP1 expression and SUVmax (P = .003). SUVmax was higher in patients with poorly differentiated HCC (mean, 6.7 ± 3.6 [standard deviation]) than in those with well- (mean, 2.6 ± 0.7, P < .001) or moderately (mean, 4.1 ± 2.3, P < .001) differentiated HCC. FBP1 expression was significantly lower in patients with poorly differentiated HCC (mean, 0.6 ± 0.2) than in those with well- (mean, 1.4 ± 0.6, P = .006) or moderately (mean, 1.2 ± 0.2, P = .007) differentiated HCC. FBP1 overexpression in HCC cells led to a significant decrease in GLUT1 expression (P = .034), 18F FDG uptake (P = .023), and HIF1A transcriptional activity (P = .001). Conclusion SUVmax in patients with HCC is inversely associated with FBP1 expression, and FBP1 may inhibit 18F FDG uptake via the HIF1A pathway. SUVmax is higher in patients with poorly differentiated HCC than in those with well- or moderately differentiated HCC, which could be the result of lower FBP1 expression in the former. © RSNA, 2017. |
Databáze: | OpenAIRE |
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