Optimization of Aqueous Extraction from Kalanchoe pinnata Leaves to Obtain the Highest Content of an Anti-inflammatory Flavonoid using a Response Surface Model
| Autor: | Bartira Rossi-Bergmann, Luana Beatriz dos Santos Nascimento, Marcela A. S. Coutinho, Marcos Vinicius Leal-Costa, Sônia Soares Costa, Eliana Schwartz Tavares, Paula Fernandes de Aguiar, Maria Paula Gonçalves Borsodi |
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| Rok vydání: | 2018 |
| Předmět: |
Flavonoid
Plant Science 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Drug Discovery Bryophyllum pinnatum Response surface methodology chemistry.chemical_classification Chromatography biology 010405 organic chemistry Extraction (chemistry) General Medicine Factorial experiment Kalanchoe biology.organism_classification 0104 chemical sciences Crassulaceae 010404 medicinal & biomolecular chemistry Complementary and alternative medicine chemistry Molecular Medicine Quercetin Food Science |
| Zdroj: | Phytochemical Analysis. 29:308-315 |
| ISSN: | 0958-0344 |
| Popis: | Introduction The medicinal plant Kalanchoe pinnata is a phenolic-rich species used worldwide. The reports on its pharmacological uses have increased by 70% in the last 10 years. The leaves of this plant are the main source of an unusual quercetin-diglycosyl flavonoid (QAR, quercetin arabinopyranosyl rhamnopyranoside), which can be easily extracted using water. QAR possess a strong in vivo anti-inflammatory activity. Objective To optimize the aqueous extraction of QAR from K. pinnata leaves using a three-level full factorial design. Material and methods After a previous screening design, time (x1 ) and temperature (x2 ) were chosen as the two independent variables for optimization. Freeze-dried leaves were extracted with water (20% w/v), at 30°C, 40°C or 50°C for 5, 18 or 30 min. QAR content (determined by HPLC-DAD) and yield of extracts were analyzed. The optimized extracts were also evaluated for cytotoxicity. Results The optimal heating times for extract yield and QAR content were similar in two-dimensional (2D) surface responses (between 12.8 and 30 min), but their optimal extraction temperatures were ranged between 40°C and 50°C for QAR content and 30°C and 38°C for extract yield. A compromise region for both parameters was at the mean points that were 40°C for the extraction temperature and 18 min for the total time. Conclusion The optimized process is faster and spends less energy than the previous one (water; 30 min at 55°C); therefore is greener and more attractive for industrial purposes. This is the first report of extraction optimization of this bioactive flavonoid. Copyright © 2018 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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