Corrigendum: Partial antimicrobial and biochemical profiles of Agelenopsis naevia Walckenaer, 1842 (Araneae: Agelenidae) venom
| Autor: | I. S. Ndams, D. M. Shehu, J. Ahmed |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gel electrophoresis biology Chemistry Venom Bacillus subtilis Venom Protein biology.organism_classification Antimicrobial complex mixtures Molecular biology 03 medical and health sciences 030104 developmental biology Insect Science Candida albicans Polyacrylamide gel electrophoresis Ecology Evolution Behavior and Systematics EC50 |
| Zdroj: | Arachnology. 18:257 |
| ISSN: | 2050-9928 |
| DOI: | 10.13156/arac.2019.18.3.257 |
| Popis: | The study was carried out to determine antimicrobial and haemolytic activities as well as to determine molecular weight breadth of Agelenopsis naevia venom components. Venom was extracted by micro-dissection. To measure the haemolytic activity, venom was incubated with 1% fresh human erythrocytes in 0.58 mg/ml, 2.54 mg/ml and 4.04 mg/ml for 1 hr, 2 hr, 4 hr and 6 hr while antimicrobial activity of venom was tested on Bacillus subtilis, Candida albicans, and Salmonella typhi. The venom protein molecular weights were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2DE). The results show that haemolytic activity on human erythrocytes was dose-dependent and increased with time (42.40%–52.52%) with an effective concentration (EC50) of 2.07 mg/ml. The venom also showed significant (p < 0.05) activity against Bacillus subtilis (23.5 ± 0.50) compared to the positive and negative controls; however, there was no activity against C... |
| Databáze: | OpenAIRE |
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