| Autor: |
J. Ross Fitzgerald, Pieter-Jan A. Haas, Jovanka Bestebroer, Mirjam M. Mebius, Jos A. G. van Strijp, Cindy Fevre, Carla J. C. de Haas |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Cellular Microbiology. 16:1646-1665 |
| ISSN: |
1462-5814 |
| Popis: |
Summary In order to cause colonization and invasive disease, pathogenic bacteria secrete proteins that modulate host immune defences. Identification and characterization of these proteins leads to a better understanding of the pathological pro- cesses underlying infectious and inflammatory diseases and is essential in the development of new strategies for their prevention and treatment. Current techniques to functionally characterize these proteins are laborious and inefficient. Here we describe a high-throughput functional selection strategy using phage display in order to identify immune evasion proteins. Using this technique we identified two previously uncharacterized proteins secreted by Staphylo- coccus aureus, SElX and SSL6 that bind to neutrophil surface receptors. SElX binds PSGL-1 on neutrophils and thereby inhibits the interac- tion between PSGL-1 and P-selectin, a crucial step in the recruitment of neutrophils to the site of infection. SSL6 is the first bacterial protein identified that binds CD47, a widely expressed cell surface protein recently described as an interesting target in anti-cancer therapy. Our find- ings provide new insights into the pathogenesis of S. aureus infections and support phage display as an efficient method to identify bacterial secretome proteins interacting with humoral or cellular immune components. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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