Molecular Characterization of Extended Spectrum â-lactamase and Carbapenemase Producing Klebsiella pneumoniae from a Tertiary Care Hospital
| Autor: | Ranjeeta Adhikary, Shalini Shenoy Mulki, S Joshi, Bhavana Malavalli Venkatesh, Beena Hosdurg Bhaskar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Klebsiella
Imipenem Klebsiella pneumoniae medicine.drug_class Antibiotics Critical Care and Intensive Care Medicine Meropenem Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Intensive care polycyclic compounds medicine biology business.industry 030208 emergency & critical care medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae 030228 respiratory system chemistry business Ertapenem medicine.drug |
| Zdroj: | Indian Journal of Critical Care Medicine. 23:61-66 |
| ISSN: | 1998-359X 0972-5229 |
| Popis: | Objective The extended-spectrum beta-lactamase (ESBL) and carbapenemase producing gram-negative bacteria among the members of Enterobacteriaceae are of major health concern globally. The present study was carried out to determine proportion and genetic characterization of ESBL and carbapenemase producing Klebsiella pneumoniae strains isolated from intensive care units of a tertiary care hospital. Materials and methods A total of 250 non-duplicate K. pneumoniae isolates were recovered from various clinical specimens from our intensive care units from May 2014 to May 2015. Antibiotic susceptibility testing was performed as recommended by Clinical and Laboratory Standard Institute. Phenotypic identification of ESBL and carbapenemase producing isolates were confirmed by the double-disk synergy test, modified Hodge test, imipenem and imipenem-EDTA combined test, respectively. Molecular characterization of β-lactamase genes were performed by polymerase chain reaction. Results Out of 250 Klebsiella pneumonaie, 84% isolates were ESBL producers, 66% were carbapenem resistant based on their reduced susceptibility to imipenem, meropenem and ertapenem. Among these 165 carbapenem resistant isolates, 9.7% were positive for bla NDM-1 and these isolates were also found to be positive for one or more bla genes. Co-carriage of AmpC in ESBL and carbapenem resistant isolates were 7.8% and 3.6%, respectively and were negative for bla KPC genes. Conclusion The study indicated the prevalence of ESBLs and bla NDM-1, with additional bla genes and AmpC among the K. pneumoniae isolates in our intensive care units. NDM-1 producing Enterobacteriaceae is a growing health care problem. Detection of the prevalence of antibacterial resistance pattern helps towards improved antibiotic policy and empirical antibiotic treatment. How to cite this article Beena HB, Shenoy SM, et al. Molecular Characterization of Extended Spectrum β-lactamase and Carbapenemase Producing Klebsiella pneumoniae from a Tertiary Care Hospital. Indian J of Crit Care Med 2019;23(2):61-66. |
| Databáze: | OpenAIRE |
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