Association of MalaysianHelicobacter pyloriVirulence Polymorphisms with Severity of Gastritis and Patients’ Ethnicity
| Autor: | Abdullah S. Anwar, Mohamed Rose Isa, Hanafiah Alfizah, Mohamed Ramelah, A. M. Rizal |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
education.field_of_study
biology Population Gastroenterology Virulence General Medicine Helicobacter pylori bacterial infections and mycoses biology.organism_classification digestive system diseases Microbiology Infectious Diseases Genotype Immunology medicine bacteria CagA Restriction fragment length polymorphism Gastritis medicine.symptom Allele education |
| Zdroj: | Helicobacter. 17:340-349 |
| ISSN: | 1083-4389 |
| DOI: | 10.1111/j.1523-5378.2012.00956.x |
| Popis: | Background and aim: Polymorphisms of Helicobacter pylori cagA and vacA genes do exist and may contribute to differences in H. pylori infection and gastroduodenal diseases among races in the Malaysian population. This study was conducted to characterize the polymorphisms in H. pylori cagA and vacA in Malaysian population. Methods: A total of 110 H. pylori isolates were genotyped by PCR and sequenced for cagA and PCR-RFLP for vacA. Results: East Asian cagA was predominantly detected (64.5%), whereas vacA s1m1 and s1m2 alleles were detected in 60.9 and 37.3% of strains, respectively. A statistical association between cagA type with patients’ ethnicity (p 50 years old (p = .027) was identified. vacA alleles showed significant association with age group >50 years old (p = .017) and increased neutrophil activity in gastric mucosa (p = .028 and p = .016 for moderate and marked activity, respectively). Further identification of vacA polymorphism revealed that 84% of strains from Malays and Indians showed one RFLP pattern (RFLP-1), whereas more than one RFLP patterns (RFLP-2, 3, 4, 5, 6, and 8) were predominantly observed in strains from Chinese (82%) (p |
| Databáze: | OpenAIRE |
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