Isolation and Characterization of 27 Novel Microsatellite Loci in Critically Endangered Wyoming Toad
| Autor: | Heidi Meador, Lee Bender, Renee M. Martin, Lacey R. Hopper |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Amphibian 0303 health sciences Ecology biology Endangered species Anaxyrus baxteri Zoology Isolation (microbiology) biology.organism_classification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Critically endangered Wyoming toad biology.animal Captive breeding Microsatellite Animal Science and Zoology Ecology Evolution Behavior and Systematics 030304 developmental biology Nature and Landscape Conservation |
| Zdroj: | Journal of Fish and Wildlife Management. 10:563-566 |
| ISSN: | 1944-687X |
| DOI: | 10.3996/042019-jfwm-029 |
| Popis: | Wyoming toad Anaxyrus baxteri is a federally endangered amphibian endemic to the Laramie basin in southwestern Wyoming, USA. A captive breeding program propagates A. baxteri, and the monitoring of genetic diversity in the captive stock can assist in guiding conservation measures of this species. Illumina paired-end sequencing lead to 27 species-specific polymorphic microsatellite genetic markers being developed. Across 24 samples, A. baxteri exhibited two to eight alleles per locus, and observed and expected heterozygosities per locus ranged from 0.292 to 0.958 and from 0.344 to 0.787, respectively. Tests for Hardy–Weinberg equilibrium were nonsignificant except for Abax_13 and Abax_39. These microsatellite markers will be useful for genetic monitoring to aid recovery efforts of A. baxteri captive and wild populations as well as other amphibians in the family Bufonidae. |
| Databáze: | OpenAIRE |
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