Diseases caused by reactions of T lymphocytes to incompatible structures of the major histocompatibility complex. V. High titers of IgG autoantibodies to double-stranded DNA
| Autor: | E H van Elven, F M van der Veen, A G Rolink, P Issa, T M Duin, E Gleichmann |
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| Rok vydání: | 1981 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 127:2435-2438 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.127.6.2435 |
| Popis: | IgG autoantibodies to double-stranded DNA (dsDNA), reaching a maximal serum titer of 1 in 2560, were spontaneously produced by (C57BL/10 x DBA/2)F1 mice injected with T lymphocytes from strain DBA/2. Anti-dsDNA antibodies were detected by the indirect immunofluorescence technique applying the kinetoplast dsDNA of Crithidea luciliae as antigenic substrate. The anti-dsDNA antibodies belonged to the IgG1, IgG2, IgM, and IgA (sub)classes. That of least some of them were produced by B cells of the F1 recipients was shown by the presence of F1-derived Ig-1b allotypic markers on such antibodies. In the F1 recipients, an incompatibility at H-2 was required for the formation of anti-dsDNA. These findings are consistent with the concept that the mechanism underlying the formation of SLE-like autoantibodies in this model is an abnormal cooperation between alloreactive donor T cells, presumably helper T (TH) cells, and H-2-incompatible F1 B cells. It is known from the literature that abnormal T-B cell cooperation is a unique tool for inducing vigorous primary antibody responses to antigens that carry repeating antigenic determinants on a rigid backbone and are poor immunogens or tolerogens in the absence of abnormal T-B cell cooperation. We suggest that the self-antigen dsDNA falls into the same category of structurally and immunologically peculiar antigens. |
| Databáze: | OpenAIRE |
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