Regulation of14C-cholesterol uptake from mixed micelles by organ culture of the mucosa epithelium from human small intestine: Effects of selective ACAT inhibitor
| Autor: | O. V. Mironyuk, V.S. Repin, A. G. Talalaev, L. N. Kask, Zotikov Ae, M. A. Kornyushin, A. V. Bocharov, L. Kornelius, A. V. Pokrovskii, T. G. Vishnyakova, Baranova In |
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| Rok vydání: | 1997 |
| Předmět: |
Cholesterol
General Medicine Absorption (skin) Biology Organ culture Molecular biology Micelle General Biochemistry Genetics and Molecular Biology Epithelium Small intestine chemistry.chemical_compound medicine.anatomical_structure Biochemistry chemistry medicine Cholesterol uptake lipids (amino acids peptides and proteins) Initial rate |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 123:139-143 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | The initial rate of14C-cholesterol uptake from mixed micelles is studied using organ culture of mucosal epithelium from human small intestine obtained during surgery. Intense, reproducible, and ACAT-regulated level of specific14C-cholesterol uptake is monitored during the first 3 h of culturing. The specific14C-cholesterol uptake is assessed by the difference between the total uptake of14C-cholesterol and nonspecific uptake of14C-sitosterol from the micelles. It is shown that less than 20% of absorbed14C-cholesterol is converted into cholesterol esters. C44788, a high-affinity and selective ACAT inhibitor, inhibits14C-cholesterol uptake in a dose-dependent manner and practically has no effect on14C-oleate uptake. |
| Databáze: | OpenAIRE |
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