Comparison of the Immunomodulatory Effects of Live and Heat-killed Lactobacillus pentosus S-PT84

Autor:	Takayuki Izumo, Toshihiro Maekawa, Yoshinori Kitagawa, Masayuki Ida, Yuichi Furukawa, Yoshinobu Kiso
Rok vydání:	2011
Předmět:	Health Toxicology and Mutagenesis medicine.medical_treatment Lactobacillus pentosus Biology Toxicology In vitro Microbiology Cytokine Interferon Immunology Interleukin 12 Splenocyte medicine Ingestion Interleukin 4 medicine.drug
Zdroj:	Journal of Health Science. 57:304-310
ISSN:	1347-5207 1344-9702
Popis:	Lactobacillus pentosus S-PT84, which was isolated from Kyoto pickles, enhances splenic natural killer (NK) cell activity, increases IgA production from Peyer’s patch cells and has high T-helper 1 (Th1) cytokine and type 1interferon-inducing activity. However, all of these effects were obtained by using heat-killed S-PT84. In the present study, we investigated the immunomodulatory effect of live and heat-killed S-PT84 in BALB/c mice. Unexpectedly, live S-PT84 indicated weak activity on Th1 cytokine (interleukin-12 or interferon-γ) production from splenocytes compared to heat-killed S-PT84 in vitro. Similar tendencies were also shown in S-PT84 having different cell wall thickness. Next, we compared the immunomodulatory effect after oral ingestion of each S-PT84. The results indicated splenic NK activity was equally enhanced by live or heat-killed S-PT84 ingestion, though heat-killed S-PT84 indicated slightly higher activity in interferon-γ production and inclination toward Th1 immunity. These results suggested that both live and heat-killed S-PT84 have an equal immunomodulatory effect in BALB/c mice. It is thought that the use of heat-killed S-PT84 is advantageous from the viewpoint of ease of handling compared to live S-PT84.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b87b46b0f057d9824e7358904d873cb5 https://doi.org/10.1248/jhs.57.304 Zobrazit plný text záznamu