Purification of cellobiohydrolase I fromTrichoderma reesei using monoclonal antibodies in an immunomatrix
| Autor: | D. E. Eveleigh, J. D. Macmillan, F. J. Riske |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology medicine.drug_class Elution Bioengineering Cellulase biology.organism_classification Monoclonal antibody Applied Microbiology and Biotechnology Sepharose Enzyme chemistry Affinity chromatography Biochemistry Cellobiohydrolase I medicine biology.protein Trichoderma reesei Biotechnology |
| Zdroj: | Journal of Industrial Microbiology. 1:259-264 |
| ISSN: | 1476-5535 0169-4146 |
| DOI: | 10.1007/bf01569280 |
| Popis: | The several components of the fungal cellulase system present practical problems in devising facile and efficient schemes for their purification. We report on a new single-step affinity chromatographic method for purification of cellobiohydrolase I ofTrichoderma reesei based on its selective absorption and elution using an immunomatrix constructed with CnBr-activated Sepharose 4B and monoclonal antibody specific for the enzyme. Isoenzymes of cellobiohydrolase I were purified directly from crude culture filtrate. The method is fast, simple, and of high resolution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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