| Autor: |
S. P. Mohanty, O. Basu, P. U. Devi, Christian Streffer, Friedo Zölzer |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Cell Proliferation. 43:579-583 |
| ISSN: |
0960-7722 |
| DOI: |
10.1111/j.1365-2184.2010.00707.x |
| Popis: |
Objectives: Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment. Materials and methods: Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms. Results: S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis. Conclusions: Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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