| Popis: |
Background Acute respiratory distress syndrome (ARDS) and acute lung injury are important clinical problems in intensive care units. The most common extrapulmonary organ failure in cases of ARDS is acute kidney injury (AKI). This study was designed to investigate the effects of tocilizumab and dexamethasone on antioxidant and proinflammatory cytokines in acute renal failure in rats with an oleic acid-induced ARDS model. Methods Six groups of rats were included in the research: 1) the CN group (healthy rats), 2) OA group (50 µL of oleic acid, i.v.), 3) OA+TCZ-2 group (oleic acid and tocilizumab at 2 mg/kg, i.p.), 4) OA+TCZ-4 group (oleic acid and tocilizumab at 4 mg/kg, i.p.), 5) OA+DEX-0.1 group (oleic acid and dexamethasone at 0.1 mg/kg, i.p.), and 6) OA+DEX-10 group (oleic acid and dexamethasone at 10 mg/kg, i.p.). After all rats were euthanized, kidney tissues were harvested for biochemical, histopathological, immunohistochemical, and scanning electron microscope (SEM) analysis. Results The lipid peroxidation levels in the kidneys were statistically decreased in the OA+TCZ-4 group compared to the OA group (P0.05) between the OA group and the TCZ and DEX groups, but a decrease was determined in the OA+TCZ-4 group. Histopathological findings such as atrophy/hypertrophy in the glomerulus, degeneration and necrosis in the tubular epithelium, hyaline debris in the tubule lumen, and capillary congestion were less prevalent in the OA+TCZ-4 group (PConclusions Tocilizumabtreatment at a dose of 4 mg/kg was more effective than other treatments in improving renal glomerular and tubular damage, expression levels of proinflammatory cytokines (TNF-α and IL-33), and antioxidant parameters in cases of oleic acid-induced ARDS. |
