Vascular Cell Induction Culture System Using Arabidopsis Leaves (VISUAL) Reveals the Sequential Differentiation of Sieve Element-Like Cells
| Autor: | Masato Saito, Yuki Kondo, Nobutaka Mitsuda, Kyoko Yamazaki, Hiroo Fukuda, Alif Meem Nurani, Chieko Saito, Yasunori Ichihashi, Masaru Ohme-Takagi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Cellular differentiation fungi Cell food and beverages Xylem Cell Biology Plant Science biology.organism_classification Cell biology Transcriptome 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Arabidopsis Botany medicine Arabidopsis thaliana Phloem Stem cell |
| Zdroj: | The Plant Cell. 28:1250-1262 |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | Cell differentiation is a complex process involving multiple steps, from initial cell fate specification to final differentiation. Procambial/cambial cells, which act as vascular stem cells, differentiate into both xylem and phloem cells during vascular development. Recent studies have identified regulatory cascades for xylem differentiation. However, the molecular mechanism underlying phloem differentiation is largely unexplored due to technical challenges. Here, we established an ectopic induction system for phloem differentiation named Vascular Cell Induction Culture System Using Arabidopsis Leaves (VISUAL). Our results verified similarities between VISUAL-induced Arabidopsis thaliana phloem cells and in vivo sieve elements. We performed network analysis using transcriptome data with VISUAL to dissect the processes underlying phloem differentiation, eventually identifying a factor involved in the regulation of the master transcription factor gene APL. Thus, our culture system opens up new avenues not only for genetic studies of phloem differentiation, but also for future investigations of multidirectional differentiation from vascular stem cells. |
| Databáze: | OpenAIRE |
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