Production and Identification of Biologically Active Peptides Derived from By-product of Hen Egg-Yolk Phospholipid Extraction
| Autor: | Józefa Chrzanowska, Łukasz Bobak, Tadeusz Trziszka, Agnieszka Zabłocka, Aleksandra Zambrowicz, Ewelina Eckert |
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| Rok vydání: | 2018 |
| Předmět: |
Proteases
food.ingredient Antioxidant DPPH medicine.medical_treatment Size-exclusion chromatography Bioengineering 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound food Yolk Drug Discovery medicine Serine protease biology 010405 organic chemistry Yarrowia biology.organism_classification Yeast 0104 chemical sciences chemistry biology.protein Molecular Medicine |
| Zdroj: | International Journal of Peptide Research and Therapeutics. 25:669-680 |
| ISSN: | 1573-3904 1573-3149 |
| DOI: | 10.1007/s10989-018-9713-x |
| Popis: | Biologically active peptides derived from food proteins have been increasingly popular due to their therapeutic properties. This paper attempts to utilize a by-product of phospholipid extraction from egg yolk as a source of peptides with antioxidant [radical scavenging capacity, Fe2+ chelating effect, reducing power (FRAP)] and ACE-inhibitory activity. In addition, in this research we analysed the ability to release bio-peptides of a non-commercial proteases obtained from Yarrowia lipolytica yeast. Yeast serine protease exert greater ability to evaluate antioxidant and ACE inhibitory activity than yeast aspartyl protease during degradation of protein by-product. Purification procedures including membrane filtration, gel filtration chromatography and reversed-phase high-performance liquid chromatography led to obtain a decapeptide QSLVSVPGMS with strong DPPH free radical scavenging and ACE inhibitory activities. A new application of the serine protease from Y. lipolytica yeast in the production of bioactive peptides from egg yolk protein by-product was demonstrated. |
| Databáze: | OpenAIRE |
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