Caspase-11 Contributes to Oviduct Pathology during Genital Chlamydia Infection in Mice
| Autor: | Uma M. Nagarajan, John A. Allen, Angela Lovett, Manoj K. Tripathy, Yugen Zhang, Stephanie A. Montgomery, Clare E. Gyorke |
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| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty animal structures Chlamydia Transgene Immunology Uterus Caspase-11 Biology medicine.disease_cause medicine.disease biology.organism_classification Microbiology Infectious Diseases medicine.anatomical_structure medicine Oviduct Parasitology Chlamydia trachomatis Chlamydia muridarum Pathogen |
| Zdroj: | Infection and Immunity. 87 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00262-19 |
| Popis: | It has been shown that caspase-1, but not its upstream activator, ASC, contributes to oviduct pathology during mouse genital Chlamydia muridarum infection. We hypothesized that this dichotomy is due to the inadvertent absence of caspase-11 in previously used caspase-1-deficient mice. To address this, we studied the independent contributions of caspase-1 and -11 during genital Chlamydia infection. Our results show that caspase-11 deficiency was sufficient to recapitulate the effect of the combined absence of both caspase-1 and caspase-11 on oviduct pathology. Further, mice that were deficient for both caspase-1 and -11 but that expressed caspase-11 as a transgene (essentially, caspase-1-deficient mice) had no significant difference in oviduct pathology from control mice. Caspase-11-deficient mice showed reduced dilation in both the oviducts and uterus. To determine the mechanism by which caspase-11-deficient mice developed reduced pathology, the chlamydial burden and immune cell infiltration were determined in the oviducts. In the caspase-11-deficient mice, we observed increased chlamydial burdens in the upper genital tract, which correlated with increased CD4 T cell recruitment, suggesting a contribution of caspase-11 in infection control. Additionally, there were significantly fewer neutrophils in the oviducts of caspase-11-deficient mice, supporting the observed decrease in the incidence of oviduct pathology. Therefore, caspase-11 activation contributes to pathogen control and oviduct disease independently of caspase-1 activation. |
| Databáze: | OpenAIRE |
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