Knotting dynamics during DNA replication
| Autor: | Pablo E. Hernández, Leticia Olavarrieta, María Luisa Martínez-Robles, Dora B. Krimer, Jorge Bernardo Schvartzman |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
biology Topoisomerase DNA replication food and beverages Mathematics::Geometric Topology Microbiology chemistry.chemical_compound surgical procedures operative Plasmid stomatognathic system chemistry Plasmid dna Agarose gel electrophoresis Biophysics biology.protein DNA supercoil Molecular Biology DNA S phase |
| Zdroj: | Molecular Microbiology. 46:699-707 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1046/j.1365-2958.2002.03217.x |
| Popis: | The topology of plasmid DNA changes continuously as replication progresses. But the dynamics of the process remains to be fully understood. Knotted bubbles form when topo IV knots the daughter duplexes behind the fork in response to their degree of intertwining. Here, we show that knotted bubbles can form during unimpaired DNA replication, but they become more evident in partially replicated intermediates containing a stalled fork. To learn more about the dynamics of knot formation as replication advances, we used two-dimensional agarose gel electrophoresis to identify knotted bubbles in partially replicated molecules in which the replication fork stalled at different stages of the process. The number and complexity of knotted bubbles rose as a function of bubble size, suggesting that knotting is affected by both precatenane density and bubble size. |
| Databáze: | OpenAIRE |
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