Functionalization of multiwalled carbon nanotubes by microwave irradiation for lysozyme attachment: comparison of covalent and adsorption methods by kinetics of thermal inactivation
| Autor: | Teresa del Castillo-Castro, D.E. Rodríguez-Félix, M. M. Castillo-Ortega, Daniel Puentes-Camacho, Enrique F. Velázquez, Rogerio R. Sotelo-Mundo |
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| Rok vydání: | 2017 |
| Předmět: |
Nanotube
Materials science Biocompatibility Kinetics 02 engineering and technology Carbon nanotube 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Industrial and Manufacturing Engineering 0104 chemical sciences law.invention chemistry.chemical_compound Adsorption chemistry Chemical engineering law Covalent bond Polymer chemistry Surface modification General Materials Science Electrical and Electronic Engineering Lysozyme 0210 nano-technology |
| Zdroj: | Advances in Natural Sciences: Nanoscience and Nanotechnology. 8:045011 |
| ISSN: | 2043-6262 |
| DOI: | 10.1088/2043-6254/aa8b3c |
| Popis: | Proteins suffer changes in their tertiary structure when they are immobilized, and enzymatic activity is affected due to the low biocompatibility of some supporting materials. In this work immobilization of lysozyme on carbon nanotubes previously functionalized by microwave irradiation was studied. The effectiveness of the microwave-assisted acid treatment of carbon nanotubes was evaluated by XPS, TEM, Raman and FTIR spectroscopy. The carboxylic modification of nanotube surfaces by this fast, simple and feasible method allowed the physical adsorption and covalent linking of active lysozyme onto the carbonaceous material. Thermal inactivation kinetics, thermodynamic parameters and storage stability were studied for adsorbed and covalent enzyme complexes. A major stability was found for lysozyme immobilized by the covalent method, the activation energy for inactivation of the enzyme was higher for the covalent method and it was stable after 50 d of storage at 4 ?C. The current study highlights the effect of protein immobilization method on the biotechnological potential of nanostructured biocatalysts. |
| Databáze: | OpenAIRE |
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