Establishment and Characterization of a Stable, Amplified Chinese Hamster Ovary (CHO) Cell Line Producing human Insulin-like Growth Factor Binding Protein-1 (hIGFBP-1)

Autor:	C. Dyring, K. Mellström, M. Lake
Rok vydání:	1995
Předmět:	Expression vector biology Chemistry Growth factor medicine.medical_treatment Binding protein Chinese hamster ovary cell Molecular biology Complementary DNA Dihydrofolate reductase medicine biology.protein Gene Intracellular
Zdroj:	Animal Cell Technology: Developments Towards the 21st Century ISBN: 9789401041959
DOI:	10.1007/978-94-011-0437-1_3
Popis:	Stable expression of human Insulin-like growth factor binding protein-1 (IGFBP-1), as determined by a biosensor, the BIACore, at high levels has been achieved in Chinese Hamster Ovary (CHO) cells by cotransfection and subsequent coamplification of expression vectors containing the IGFBP-1 cDNA and a dihydrofolate reductase (DHFR) cDNA gene into CHO DHFR-deficient cells. Stepwise selection of the DHFR+ transformants in increasing concentrations of methotrexate generated cells with a high copy number of IGFBP-1 genes. IGFBP-1 expression was found to increase withincreasing copy number. These cells werestably amplified and expressed high levels ofhuman IGFBP-1 in the absence of MTX. Aflow cytometric method was used to measure the intracellular level of IGFBP-1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b834e64f03b2ccef131b198f7089c3d5 https://doi.org/10.1007/978-94-011-0437-1_3 Zobrazit plný text záznamu