Characterization of Porcine Endometrial, Myometrial, and Mammary Oxytocin Binding Sites during Gestation and Labor1

Autor:	T I Koike, Murray D. Mitchell, R.W. Rorie, D. L. Kreider, D. Hardesty, S. Lundin-Schiller
Rok vydání:	1996
Předmět:	Estrous cycle medicine.medical_specialty Receptor expression Myometrium Uterus Radioimmunoassay Cell Biology General Medicine Biology Endometrium medicine.anatomical_structure Endocrinology Reproductive Medicine Oxytocin Internal medicine medicine Binding site medicine.drug
Zdroj:	Biology of Reproduction. 55:575-581
ISSN:	1529-7268 0006-3363
Popis:	Changes in oxytocin (OT) receptor expression have been found to be an important determinant of the physiological effect of OT in several species. To date there are no published studies of OT binding sites during pregnancy in the pig. The purpose of the present study is to improve understanding of the role of OT in porcine parturition. The concentration and affinity of OT binding sites were determined for myometrium and endometrium from pregnant and postpartum gilts. Tissues were obtained after slaughter from 7 animals in each of four groups: 1) 90 days gestation, 2) 112 days gestation, 3) term after milk letdown (before delivery), and 4) within 1-3 h after farrowing. Mammary tissues were obtained for some animals in each group (n = 3-5/group). Before slaughter, blood was collected from each animal and assayed for estradiol-17 beta, progesterone, 13,14-dihydro-15-keto-prostaglandin F-2 alpha (PGFM), and OT. Binding of H-3-OT in the three tissues was concentration- and time-dependent. Sites of H-3-OT binding (fmol/mg protein +/- SEM) increased toward term for each tissue and remained elevated in the postpartum group. Endometrial and mammary tissues displayed the most acute increases in OT binding site concentrations while myometrial tissues displayed a more gradual increase in OT binding sites over the times studied. The binding sites displayed high affinity for H-3-OT and were characterized by linear Scatchard plots. Concentrations of estradiol-17 beta, PGFM, and OT (pg/ml +/- SEM) were positively correlated with H-3-OT binding site concentrations, whereas progestrone concentrations (ng/ml +/- SEM) were negatively correlated with binding site concentration, as determined by Pearson's Correlation Analysis. The data represent the first account of changes in the expression of OT binding sites on porcine tissues during gestation and labor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b81a4012dfabb7751c922163fbe00818 https://doi.org/10.1095/biolreprod55.3.575 Zobrazit plný text záznamu