Identification and Variability of the GDP-L-Galactose Phosphosphorylase Gene ApGGP1 in Leek Cultivars
| Autor: | Anna V. Shchennikova, Elena Z. Kochieva, M. A. Filyushin, Olga K. Anisimova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences biology food and beverages biology.organism_classification Allium porrum 01 natural sciences White (mutation) 03 medical and health sciences chemistry.chemical_compound chemistry Polymorphism (computer science) Galactose Gene expression Botany Genetics Allium Cultivar Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Russian Journal of Genetics. 57:311-318 |
| ISSN: | 1608-3369 1022-7954 |
| DOI: | 10.1134/s1022795421030030 |
| Popis: | The ApGGP1 coding sequences, encoding GDP-L-galactose phosphorylase, the key enzyme of the L-galactose pathway of vitamin C biosynthesis, have been identified and characterized in a wide range of leek (Allium porrum L.) cultivars, differing in vitamin C content. Analysis of ApGGP1 polymorphism in 35 leek cultivars revealed 29 allelic variants, corresponding to 11 variants of the ApGGP1 protein. It was found that leek and other Allium species ApGGP1 sequences contained a specific C-terminal motif (50 a.a.). The ApGGP1 expression pattern was determined in various leek organs and tissues (roots, stem, white pseudo stem, and green leaves (young and mature)). ApGGP1 transcripts were detected in all analyzed tissues. The maximum expression level was observed in leaves, and the minimum was in stem. The level of gene expression in roots (non-photosynthetic tissue) was quite high and comparable with that in young green leaves. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink