Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes
| Autor: | P. Caunt, A. Impoolsup, P.F. Greenfield |
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| Rok vydání: | 1989 |
| Předmět: |
Strain (chemistry)
Saccharomyces cerevisiae Bioengineering General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Enterobacteriaceae Yeast law.invention Microbiology Plasmid Biochemistry law Gene expression Recombinant DNA medicine Escherichia coli Biotechnology |
| Zdroj: | Biotechnology Letters. 11:605-608 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01025266 |
| Popis: | The 2μm based yeast plasmid, pLG669-z, was stably maintained in a S. cerevisiae strain during continuous culture with cyclic growth rate changes. In the absence of a selective mechanism, the fraction of plasmid containing cells remained high, and even without optimisation, high gene expression levels could be obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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