FRI0009 Decreased Frequencies of Circulating CD19+CD24highCD38highB Cells in ACPA+ Rheumatoid Arthritis (RA) Patients
| Autor: | Amaya Puig-Kröger, María-Belén Bautista-Caro, E. Martin-Mola, Chamaida Plasencia, Alejandro Villalba, María-Eugenia Miranda-Carús, Irene Arroyo-Villa, P. Aguado, Laura Nuño, M.-G. Bonilla-Hernán, Alejandro Balsa |
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| Rok vydání: | 2015 |
| Předmět: |
musculoskeletal diseases
biology business.industry CD24 CD3 Immunology hemic and immune systems CD38 medicine.disease Peripheral blood mononuclear cell General Biochemistry Genetics and Molecular Biology CD19 Rheumatology immune system diseases hemic and lymphatic diseases Rheumatoid arthritis medicine biology.protein Immunology and Allergy Rheumatoid factor Antibody skin and connective tissue diseases business |
| Zdroj: | Annals of the Rheumatic Diseases. 74:422.3-422 |
| ISSN: | 1468-2060 0003-4967 |
| Popis: | Background CD19+CD24 high CD38 high B cells have been described to have a regulatory capacity and their frequency is altered in the peripheral blood of patients with various autoimmune diseases. Objectives To determine the frequency of circulating CD19+CD24 high CD38 high B cells in patients with RA, and establish a correlation with clinical parameters. Methods Peripheral blood was drawn from RA patients treated with non-biological DMARDs (n=48), RA patients treated with anti-TNFalpha drugs (n=15) and healthy controls (n=63) that were matched with patients for age and gender. After isolation by Ficoll-Hypaque gradient, PBMCs were stained with antibodies to CD3, CD4, CD19, CD24, and CD38, and examined by flow cytometry. Results A decreased frequency of CD19+CD24 high CD38 high B cells was apparent in in ACPA+ but not ACPA- RA patients, and this was observed not only in patients treated with non-biological DMARDs but also in patients receiving anti-TNFalpha drugs. In addition, the frequency of CD19+CD24 high CD38 high B cells showed a significant negative correlation with ACPA titers (r = -0.43, p=0.023). In contrast, no correlation was found between the frequency of circulating CD19+CD24 high CD38 high B cells and either rheumatoid factor titres or disease activity as determined by the DAS28 score. Conclusions A decreased frequency of CD19+CD24 high CD38 high B cells is observed in ACPA+ but not ACPA- RA patients treated with either nonbiological DMARDs or anti-TNFalpha drugs, that is related with ACPA titres but not with rheumatoid factor titres or disease activity. Disclosure of Interest None declared |
| Databáze: | OpenAIRE |
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