Use of Cyclodextrin-Cholesterol Complex as a Primary Standard in Cholesterol Analysis
| Autor: | N. Karuppiah, S.A. Kapustka, P.B. Kaufman, A. Sharma |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Analyte Chromatography Primary (chemistry) Cyclodextrin Cholesterol medicine.medical_treatment Analytical Chemistry Steroid chemistry.chemical_compound Enzyme chemistry Biochemistry Reagent medicine lipids (amino acids peptides and proteins) Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Microchemical Journal. 47:325-329 |
| ISSN: | 0026-265X |
| Popis: | Cholesterol is an analyte commonly measured in various biological fluids. Enzymic procedures have virtually replaced the chemical methods for cholesterol determinations. Standardization of cholesterol assays often involve the use of solutions of this steroid containing organic solvents and/or detergents, or alternatively, the use of secondary standards. The present report shows the preparation of a primary cholesterol standard by its complexation with a chemically modified cyclodextrin. hydroxylpropyl β-cyclodextrin. The use of cyclodextrin for cholesterol solubilization is not deleterious to the enzymes and other constituents present in reagents for cholesterol analysis. Such a standard is biocompatible. stable, and safe and offers an excellent alternative to the utilization of organic solvents or detergents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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