A centralized model for creating shared, standardized, microsatellite data that simplifies inter-laboratory collaboration

Autor: Jon E. Hess, Ora L. Schlei, Chris Kozfkay, Matt R. Campbell, Donald M. Van Doornik, Jeff J. Stephenson, Shawn R. Narum, Melanie M. Paquin, Paul Moran, John K. Wenburg, Maureen P. Small, Andrew P. Matala, Megan V. McPhee
Rok vydání: 2008
Předmět:
Zdroj: Conservation Genetics. 10:1145-1149
ISSN: 1572-9737
1566-0621
DOI: 10.1007/s10592-008-9729-4
Popis: We demonstrate an efficient model for standardizing microsatellite DNA data among laboratories studying Oncorhynchus mykiss. Eight laboratories standardized 13 microsatellite loci following allele nomenclature of a central laboratory (average inter-laboratory genotyping concordance >98%). Following this central model, we have currently standardized 298 alleles from throughout the species native range. Although we focus here on O. mykiss, our experiences and recommendation apply equally to other broadly distributed species that may benefit from multi-laboratory collaborative data collection.
Databáze: OpenAIRE
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