Effect of Phenobarbital and Buthionine Sulfoximine Pretreatment on Aflatoxin B1-Induced Glutathione S-Transferase Placental form Positive Single Hepatocytes in Rats
| Autor: | Kiyoko Lehmann, Kojiro Tsuji, Prabhakar D. Lotlikar, Kiyomi Sato, Hisashi Shinozuka |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Aflatoxin biology Health Toxicology and Mutagenesis Glutathione Toxicology medicine.disease_cause chemistry.chemical_compound Glutathione S-transferase Endocrinology chemistry Internal medicine medicine biology.protein Immunohistochemistry Phenobarbital Buthionine sulfoximine Carcinogenesis Carcinogen medicine.drug |
| Zdroj: | Journal of Toxicology: Toxin Reviews. 8:227-235 |
| ISSN: | 0731-3837 |
| DOI: | 10.3109/15569548909059752 |
| Popis: | Using a single dose carcinogen model developed in the rat (Moore et al., Carcinogenesis 8, 483, 1987), we have examined the effects of either phenobarbital (PB) or/and buthionine sulfoximine (BSO) pretreatment of male Fischer rats on aflatoxin B1 (AFB1)-induced glutathione S-transferase placental form (GST-P) positive single hepatocytes detected by the immunohistochemical method. Control rats 48 hr. after i.p. administration of AFB1 (2 mg/kg body wt.) yielded about 10.1 ± 4.8 GST-P positive single hepatocytes/sq. cm.; PB pretreatment inhibited the generation of GST-P positive single hepatocytes to 28% of control levels. BSO pretreatment of these two groups before AFB1 injection increased the yield of GST-P positive hepatocytes by 145% and 180% of the respective controls. There appears to be a good correlation between modulation of hepatic AFB1-DNA binding by PB or/and BSO pretreatment and the presence of AFB1-induced GST-P positive single hepatocytes in rats. |
| Databáze: | OpenAIRE |
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