Epidemiological and genetic characterization ofClostridium butyricumcultured from neonatal cases of necrotizing enterocolitis in China

Autor: Fengqin Li, Di Zhang, Scott V. Nguyen, Nikunj Maheshwari, Séamus Fanning, Ying Li, Yujie Hu, Yinping Dong, Yu Cao
Rok vydání: 2020
Předmět:
Zdroj: Infection Control & Hospital Epidemiology. 41:900-907
ISSN: 1559-6834
0899-823X
Popis: Objective:Laboratory-based characterization and traceback ofClostridium butyricumisolates linked to outbreak cases of neonatal necrotizing enterocolitis (NEC) in a hospital in China.Methods:In total, 37 samples were collected during the NEC outbreak. Classical bacteriological methods were applied to isolate and identifyClostridiumspp. Meanwhile, 24 samples collected after an outbreak were similarly tested. AllClostridiumisolates were identified to species level as eitherC. butyricumorC. sporogenes. These isolates were subsequently subtyped using pulsed-field gel electrophoresis (PFGE). Genomic DNA was purified from 2 representativeC. butyricumisolates and sequenced to completion.Results:Of 37 samples collected during the NEC outbreak, 17 (45.95%) were positive forClostridiumspp. One species,C. butyricum, was cultured from 10 samples. Another species cultured from 2 other samples was identified asC. sporogenes. Both of these species were cocultured from 5 samples. Pulsotyping showed that the 15C. butyricumand the 7C. sporogenesisolates produced indistinguishable DNA profiles. No NEC cases were reported after disinfection following the outbreak, and all samples collected after the outbreak were negative forClostridiumspp. Whole-genome sequencing (WGS) indicated that sialidase, hemolysin, and enterotoxin virulence factors were located on the chromosomes of 2C. butyricumisolates.Conclusions:The outbreak of NEC was epidemiologically linked toC. butyricumcontamination within the hospital. This is the first report of an NEC outbreak associated withC. butyricuminfection in China.
Databáze: OpenAIRE
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