A QUANTITATIVE HPLC DETECTION METHOD FOR WHI-P154 [4-(3′-BROMO-4′-HYDROXYLPHENYL)-AMINO-6,7-DIMETHOXYQUINAZOLINE]

Autor:	X. P. Liu, F. M. Uckun, C. L. Chen, R. K. Narla
Rok vydání:	1999
Předmět:	Chromatography Chloroform Bicyclic molecule Chemistry Elution Clinical Biochemistry Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Chromatography detector Methanol Acetonitrile Quantitative analysis (chemistry)
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 22:1771-1783
ISSN:	1520-572X 1082-6076
Popis:	WHI-P154 [4-(3′-bromo-4′hydroxylphenyl)-amino-6,7 di-methoxyquinazoline] is a novel anti-tumor agent with unique cytotoxic activity against human glioblastoma cells (Clin. Cancer. Res. 4:1405-1414, 1998). Further development of WHI-P154 will require detailed pharmacodynamic studies in preclinical animal models. Therefore, we established a sensitive and accurate high performance liquid chromatography (HPLC)-based quantitative detection method for WHI-P154. This method allows the measurement of WHI-P154 levels in plasma, as well as in target human glioblastoma cells. Plasma and cell lysates were extracted with chloroform, dried with nitrogen gas and reconstituted in methanol : water (9:1, v/v). An aliquot was injected into a Hewlett Packard HPLC system employing a 250 × 4mm Lichrospher 100, RP-18 (5 μm) analytical column in conjunction with a 4 × 4 mm Lichrospher 100, RP-18 (5 μm) guard column. The eluted compounds were detected by a diode array detector set at a wavelength of 335 nm. Acetonitrile/water con...
Databáze:	OpenAIRE
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