The entry of sugar beet cells into the G0 state involves extensive re-programming of gene expression mechanisms via transcriptional and translational controls

Autor:	Adrian Slater, Nigel W. Scott, Malcolm Elliott, Mark R. Fowler, Melissa J. Kirby
Rok vydání:	1998
Předmět:	Messenger RNA Differential display Cell division Protein turnover Plant Science General Medicine Biology Molecular biology Cell culture Gene expression Genetics Protein biosynthesis Northern blot Agronomy and Crop Science
Zdroj:	Plant Science. 136:79-91
ISSN:	0168-9452
Popis:	Sugar beet cells grown in liquid suspension culture entered the stationary phase on day 17 of the batch culture cycle as a consequence of nutrient depletion. Between days 17 and 19 the cells exhibited a number of marked changes in RNA and protein metabolism characteristic of entry into a quiescent or G0 state. Protein synthesis declined considerably and very few polypeptides incorporated sufficient radioactively labelled methionine to be detected by fluorography following polyacrylamide gel electrophoresis (SDS-PAGE). There was a marked decrease in protein half life during the stationary phase and this was accompanied by a rise in protease activity. RNA synthesis also decreased to
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b78916801833bb8a8b92ab20e8ee909b https://doi.org/10.1016/s0168-9452(98)00117-4 Zobrazit plný text záznamu Full Text from ScienceDirect