SMAD 3 expression and regulation of fibroplasia in vocal fold injury
| Autor: | Peter A. Benedict, Iv Kraja, Ryan C. Branski, Nao Hiwatashi, Renjie Bing, Gregory R. Dion, Milan R. Amin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene knockdown Small interfering RNA Pathology medicine.medical_specialty integumentary system biology business.industry Transforming growth factor beta medicine.disease Andrology 03 medical and health sciences 030104 developmental biology Otorhinolaryngology Downregulation and upregulation Fibrosis In vivo biology.protein medicine Signal transduction business Transforming growth factor |
| Zdroj: | The Laryngoscope. 127:E308-E316 |
| ISSN: | 0023-852X |
| Popis: | Objective Recent reports highlight the efficacy of small interfering RNA (siRNA) targeting SMAD3 to regulate transforming growth factor β (TGF-β)-mediated fibroplasia in vocal fold fibroblasts. The current study sought to investigate SMAD3 expression during wound healing in vivo and quantify the downstream transcriptional events associated with SMAD3 knockdown in vitro. Study Design In vivo and in vitro. Methods Unilateral vocal fold injury was created in a rabbit model. SMAD3 and SMAD7 mRNA expression was quantified at 1 hour and 1, 3, 7, 14, 30, 60, and 90 days following injury. In vitro, multi-gene analysis technology was employed in our immortalized human vocal-fold fibroblast cell line following TGF-β1 stimulation ± SMAD3 knockdown across time points. Results SMAD3 mRNA expression increased following injury; upregulation was significant at 3 and 7 days compared to control (both P |
| Databáze: | OpenAIRE |
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