Extraction and Amplification of 16S rRNA Genes from Deep Marine Sediments and Seawater to Assess Bacterial Community Diversity

Autor: J. A. K. Will, R. J. Parkes, C. M. Turley, AJ Weightman, JC Fry, PA Rochelle, G. J. S. Jenkins
Rok vydání: 1995
Předmět:
Zdroj: Nucleic Acids in the Environment ISBN: 9783540580690
DOI: 10.1007/978-3-642-79050-8_11
Popis: Deep marine sediments remain poorly studied with respect to bacterial communities inhabiting them. Sediments up to 500 m below the sea floor (mbsf) in the Japan Sea have been laid down over the last 3 million years and therefore represent the early stages of sediment diagenesis. These sediments contain substantial bacterial populations (1.1 × 107 to 1.4 × 109 bacteria/g wet weight) with surprisingly high activity (Cragg et al. 1992; Getliff et al. 1992). Better knowledge of the types and diversity of bacteria which are dominant in these sediments, particularly those predominantly involved in important biogeochemical and biochemical processes, will lead to increased understanding of the diagenesis and evolution of organic matter. This unique habitat might also contain bacteria not found elsewhere in the biosphere.
Databáze: OpenAIRE