Isolation and first draft genome sequence of a linezolid-dependentStaphylococcus aureusclinical strain
| Autor: | Chris Baysdorfer, Beatrice Hervé, Víctor Antonio García-Angulo, Camila Azócar Sanhueza, Joel Melo, Luis Lozano Aguirre, Sebastián de la Fuente, María Teresa Ulloa |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
medicine.drug_class SCCmec Antibiotics Virulence Pathogenic bacteria biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses medicine.disease_cause Microbiology chemistry.chemical_compound Antibiotic resistance chemistry Staphylococcus aureus 23S ribosomal RNA Linezolid medicine |
| Zdroj: | Future Microbiology. 15:1123-1129 |
| ISSN: | 1746-0921 1746-0913 |
| Popis: | Background: Antibiotic-dependent pathogenic bacteria are sporadically isolated from patients that received prolonged antibiotic treatments. Evolution of antibiotics dependence and its clinical implications are scarcely studied. Materials & methods: A linezolid-dependent Staphylococcus aureus strain was isolated from a cystic fibrosis patient. A draft genome sequence was obtained and searched for known antibiotics resistance determinants and virulence factors. Results: The genome was assembled into 79 contigs for a total of 2.83 Mbp. This strain is a sequence type 5 methicillin-resistant Staphylococcus aureus with a type I SCC mec cassette also conserving the Panton–Valentine leukocidin. The G2576T substitution, conferring linezolid resistance, was harbored by all five copies of the 23S rRNA. Conclusion: The linezolid-dependent strain is related to a strain circulating in Latin America that acquired a mutation conferring linezolid resistance. |
| Databáze: | OpenAIRE |
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