Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment

Autor:	Xiao jue Guo, Zhi yan Lu, Yong Di Liu, Zhong zi Huang, Hui Li, Ping Wang, Kuang Fei Lin
Rok vydání:	2014
Předmět:	Catechol Chromatography Biodegradation Ectoine Biology Microbiology Halophile Biomaterials Metabolic pathway chemistry.chemical_compound chemistry Biochemistry Phenol Osmotic pressure Osmoprotectant Waste Management and Disposal
Zdroj:	International Biodeterioration & Biodegradation. 94:115-120
ISSN:	0964-8305
DOI:	10.1016/j.ibiod.2014.07.003
Popis:	A moderately halophilic bacterial enrichment was able to degrade 120 mg/L of phenol in the presence of 1–2 M of NaCl within 3 d or 2.5–3 M of NaCl within 6 d. The optimal degradation was achieved at 1.5 M of NaCl and 350 mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b7673b54d362b952e38acb69ddcea117 https://doi.org/10.1016/j.ibiod.2014.07.003 Zobrazit plný text záznamu Full Text from ScienceDirect