Verifying an F1screen for identification and quantification of rareBacillus thuringiensisresistance alleles in field populations of the sugarcane borer,Diatraea saccharalis

Autor: David A. Andow, Karla Emfinger, Steven Moore, Fangneng Huang, Donna R. Lee, Bisong Yue, Roy Parker, Don Cook, B. Rogers Leonard
Rok vydání: 2008
Předmět:
Zdroj: Entomologia Experimentalis et Applicata. 129:172-180
ISSN: 1570-7458
0013-8703
DOI: 10.1111/j.1570-7458.2008.00761.x
Popis: Using an F 1 screen, 352 feral individuals of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), were examined for the presence of Bacillus thuringiensis (Bt)-resistance alleles. These insects represented four geographical populations collected in central and northeastern Louisiana, USA, and one field population from the Gulf Coast area of Texas, USA, during 2006. The F 1 screen used various crosses between field-collected insects and a laboratory strain of CrylAb-resistant D. saccharalis, including both reciprocal crosses and group mating. F 1 neonates of the crosses were screened for Bt resistance on Bt maize leaf tissue. One field-collected individual of D. saccharalis was shown to have a Bt-resistance allele. Based on Bayesian analysis procedures, the Bt-resistance allele frequency in the five populations of D. saccharalis was 0.0028 with a 95% confidence interval of 0.0003-0.0079. The successful identification of a resistance allele in a field collection of insects suggests that the F 1 screening technique could be an effective tool for detecting and monitoring rare Bt-resistance alleles in field populations of D. saccharalis.
Databáze: OpenAIRE
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