Immuno‐chemical non‐cross‐reactivity between eukaryotic and prokaryotic seryl‐tRNA synthetases
| Autor: | O I Gudzera, Beresten' Sf, Michael Tukalo, L. L. Sidorik, V A Dragovoz |
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| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
biology Biophysics Cell Biology medicine.disease_cause Biochemistry Cross-reactivity Molecular biology chemistry.chemical_compound Enzyme chemistry Structural Biology Polyclonal antibodies Transfer RNA Genetics medicine biology.protein PMSF Bovine serum albumin Molecular Biology Escherichia coli Gene |
| Zdroj: | FEBS Letters. 292:76-78 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(91)80838-t |
| Popis: | Monospecific polyclonal antibodies (pAbs) against highly purified bovine seryl-tRNA synthetase (SerRS, EC 6.1.1.1) were prepared and their specificity tested. The interactions of pAbs with SerRS from different organisms were investigated by protein immunoblotting and ELISA methods. pAbs inhibit eukaryotic SerRS aminoacylating activity and exert no effect on SerRS activity from prokaryotes. It is proposed that prokaryotic and eukaryotic SerRS evolve from different ancestor genes. |
| Databáze: | OpenAIRE |
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