Hydrogel with peroxidase-like activity to enhance sensitivity of electrochemical immnuosensor
| Autor: | Zhanfang Ma, Xuanze Meng, Yang Xu, Bochen Ma, Hongliang Han |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Metals and Alloys Substrate (chemistry) 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Electrochemistry 01 natural sciences Redox 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Catalysis Chemical engineering Specific surface area Self-healing hydrogels Polyoxometalate Materials Chemistry Electrical and Electronic Engineering 0210 nano-technology Instrumentation Zeolitic imidazolate framework |
| Zdroj: | Sensors and Actuators B: Chemical. 344:130272 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2021.130272 |
| Popis: | The improvement of sensitivity was still an urgent need for electrochemical immunosensors. Aiming to satisfy the demand, it was an advantageous way to introduce catalytic hydrogel sensing interface into the immunosensor. Due to its large specific surface area and porous structure, the hydrogel can be post-doped to embed a large amount of catalysts, so that the hydrogel was endowed with catalytic performance. However, the catalytic hydrogel was usually composed of hydrogel and post-modified catalyst such as Pt nanoparticles and Pd nanoparticles, which limited the wide application of such hydrogels. In this work, the hydrogel was mainly composed of cobalt ions and polyoxometalate precursor solution with catalytic activity. Immunoprobe based on xanthine oxidase (XOD) encapsulated by zeolitic imidazolate frameworks (ZIFs) was combined with catalytic hydrogel to establish cascade reaction which effectively strengthened the electron transfer efficiency of electrochemical signal substances. The hydrogen peroxide produced by enzyme and substrate was catalyzed by the hydrogel which obviously accelerated the oxidation reaction of thinione, corresponding to significant current response. Carbohydrate antigens 72-4 (CA72-4) was selected as a model analyte to verify the amelioration of sensor. Under ideal conditions, the manufactured immunosensor had a lower detection limit 8.133 μU mL−1 and a wider detection ranging from 1.000 × 10−5 U mL−1 to 100 U mL−1. Considering the superior performance of this method, it had a promising application prospect in the field of immunosensors. |
| Databáze: | OpenAIRE |
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