Vestibular Schwann cells are a distinct subpopulation of peripheral glia with specific sensitivity to growth factors and extracellular matrix components
| Autor: | Alain Sans, Stéphanie Ventéo, Cécile Travo, Sylvain Bartolami, Marlies Knipper, Christelle Augé |
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| Rok vydání: | 2003 |
| Předmět: |
Vestibular system
0303 health sciences biology General Neuroscience Schwannoma medicine.disease Vestibular nerve Myelin basic protein Extracellular matrix 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine.anatomical_structure nervous system Laminin otorhinolaryngologic diseases biology.protein Glial cell line-derived neurotrophic factor medicine Inner ear sense organs Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Journal of Neurobiology. 57:270-290 |
| ISSN: | 0022-3034 |
| DOI: | 10.1002/neu.10302 |
| Popis: | Vestibular nerve Schwann cells are predisposed to develop schwannoma. While knowledge concerning this condition has greatly improved, little is known about properties of normal vestibular Schwann cells. In an attempt to understand this predisposition, we evaluated cell density regulation and proliferative features of these cells taken from 6-day-old rats. Data were compared to those obtained with sciatic Schwann cells. In both vestibular and sciatic 7-day-old cultures, Schwann cells appear as bipolar or flattened cells. However, sciatic and vestibular cells greatly differ in other aspects: on poly-L-lysine coating, sciatic cells specifically synthesize myelin basic protein, while expression of P0 mRNAs is restricted to some vestibular cells. Laminin increases sciatic cell density but not that of vestibular cells. Fibronectin selectively enhances the proliferation of vestibular Schwann cells and lacks an effect on sciatic ones. Comparison of cell density changes between sciatic and vestibular cells shows that they are sensitive to two different sets of growth factors. Progesterone and FGF-2 combined with forskolin selectively enhance the cell density of sciatic glia, while IGF-1 and GDNF specifically increase vestibular cell density. Furthermore, BrdU incorporation assays indicate that GDNF is also a mitogen for vestibular cells. Altogether, vestibular Schwann cells display phenotypic features and responsiveness to exogenous signals that are significantly different from sciatic Schwann cells, suggesting that vestibular glia form a subpopulation of Schwann cells. © 2003 Wiley Periodicals, Inc. J Neurobiol 57: 270–290, 2003 |
| Databáze: | OpenAIRE |
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