Isolation and characterization of galactomannan from seed of Leucaena leucocephala
| Autor: | Mohd Radzi Ahmad, Abd Rashid Li, Dzaraini Kamarun, Nurnadiah Rahim |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Polymers and Plastics
Polysaccharide 01 natural sciences High-performance liquid chromatography Hydrolysis Galactomannan chemistry.chemical_compound 0404 agricultural biotechnology Materials Chemistry skin and connective tissue diseases Derivatization chemistry.chemical_classification Leucaena leucocephala Chromatography biology 010401 analytical chemistry Water extraction 04 agricultural and veterinary sciences General Chemistry bacterial infections and mycoses Condensed Matter Physics biology.organism_classification 040401 food science 0104 chemical sciences carbohydrates (lipids) chemistry Biochemistry Galactose |
| Zdroj: | Polymer Bulletin. 75:2027-2037 |
| ISSN: | 1436-2449 0170-0839 |
| DOI: | 10.1007/s00289-017-2135-7 |
| Popis: | Seeds from Leucaena leucocephala or commonly known as white leadtree are rich in galactomannan, a reserved polysaccharide composed of d-mannose and d-galactose residues. Galactomannan was isolated from the mature seeds of L. leucocephala using water extraction followed by precipitation with ethanol and purified using ion-exchange chromatography. Pure galactomannan was successfully isolated with a yield of 50.31% (w/w). It was chemically analyzed by UV, NMR, FT-IR, GPC, and HPLC. The polysaccharide was further hydrolyzed and derivatized with 3-methyl-1-phenyl-5-pyrazolone (PMP) for determination of its monosaccharide composition using a pre-column derivatization HPLC–UV technique. It consisted of d-mannose and d-galactose with mannose:galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. FT-IR analysis, 1H, and 13C NMR of the galactomannan produced are similar to those reported in the literatures. |
| Databáze: | OpenAIRE |
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