Imaging and Time-Resolved Spectroscopy of Single Molecules at an Interface

Autor:	Louis E. Brus, Timothy D. Harris, Jay K. Trautman, J. J. Macklin
Rok vydání:	1996
Předmět:	Multidisciplinary Chemistry business.industry Transition dipole moment Fluorescence Molecular physics law.invention Dipole Optics Optical microscope law Microscopy Spontaneous emission Boundary value problem Time-resolved spectroscopy business
Zdroj:	Science. 272:255-258
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.272.5259.255
Popis:	Far-field microscopy was used to noninvasively measure the room-temperature optical properties of single dye molecules located on a polymer-air interface. Shifts in the fluorescence spectrum, due to perturbation by the locally varying molecular environment, and the orientation of the transition dipole moment were correlated to variation in the excited-state lifetime. The lifetime dependence on spectral shift is argued to result from the frequency dependence of the spontaneous emission rate; the lifetime dependence on dipole orientation was found to be a consequence of the electromagnetic boundary conditions on the fluorescent radiation at the polymer-air interface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b748c4e116c6d64ecab0144601a3f24c https://doi.org/10.1126/science.272.5259.255 Zobrazit plný text záznamu Plný text ve formátu PDF