Determining Temporal and Spatial Expression of Calpains in Amphibians
| Autor: | Neophytos Christodoulou, Sara Zanardelli, Ioanna Antoniades, Anna Charalambous, Paris A. Skourides |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Proteases medicine.diagnostic_test Xenopus Calpain In situ hybridization Biology Immunofluorescence biology.organism_classification Cell biology 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis medicine Calpain-2 biology.protein Intracellular 030304 developmental biology Cysteine |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493989874 |
| DOI: | 10.1007/978-1-4939-8988-1_6 |
| Popis: | Calpains are a family of calcium-dependent intracellular cysteine proteases that regulate important physiological processes by substrate cleavage. Despite the fact that Calpains have been identified in the Xenopus genome, their expression patterns and role have not been characterized. Therefore, herein, we describe two methods to determine temporal and spatial expression of Calpain 2 during Xenopus development, namely, RT-PCR and whole-mount in situ hybridization (WISH). In addition, indirect immunofluorescence (IF) is described to determine translocation to the plasma membrane, which correlates with activity levels of Calpain 2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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