Structure of the O-specific polysaccharide of Proteus penneri 71 and classification of cross-reactive P. penneri strains to a new proposed serogroup O64

Autor: Krystyna Zych, Alexander S. Shashkov, Zygmunt Sidorczyk, Yuriy A. Knirel, Nina A. Kocharova, Filip V. Toukach, Dominika Kaminska, Marcin Kowalczyk
Rok vydání: 2000
Předmět:
Zdroj: European Journal of Biochemistry. 267:808-814
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2000.01059.x
Popis: A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and 1H- and 13C-NMR spectroscopic studies, including two-dimensional COSY, 13C,1H heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established: 3)-β-d-GlcpNAc-(14)-β-d-GlcpNAc-(13)-α-d-Galp-(1 The polysaccharide has the same carbohydrate backbone as the O-specific polysaccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.
Databáze: OpenAIRE