| Autor: |
Ingo Klimant, Birgit Ungerböck, Torsten Mayr, Sergey M. Borisov, Günter Mistlberger |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Limnology and Oceanography: Methods. 10:101-109 |
| ISSN: |
1541-5856 |
| Popis: |
A simple and universal method to minimize optical cross-talk in chemical imaging with planar optodes used for metabolic investigation of benthic interfaces is presented. Cross-talk can lead to smeared images and flattened gradients and therefore to erroneous analytical results. To minimize the cross-talk effect, an additional optical filter layer is coated on the downside of the transparent support. Cross-talk light traveling in the support and decoupled by scattering media is absorbed to a higher extent than the virtual signal. The extent of cross-talk and its reduction was evaluated by imaging the luminescence intensity of sensor layers with sharp edges and isolated points. The influence of cross-talk on the accuracy of oxygen determination by lifetime measurements was investigated by analyzing aerated and de-aerated zones of a planar optode placed on a scattering media. The presented method is compared qualitatively with methods previously described to reduce optical cross-talk effects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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