158 BOVINE HERPES VIRUS 4 (BoHV4) INHIBITS BOVINE ENDOMETRIAL EPITHELIAL CELL (bEEC) PROLIFERATION
| Autor: | Gunilla Blomqvist, Jean-Francois Valarcher, Gaetano Donofrio, Yongzhi Guo, Pierrette Reinaud, P. Chantaraprateep, Patrice Humblot, M. Chanrot, Mikael Juremalm, Renée Båge, Gilles Charpigny, Olivier Sandra |
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| Rok vydání: | 2015 |
| Předmět: |
2. Zero hunger
0303 health sciences 030219 obstetrics & reproductive medicine Serial dilution DNA virus Embryo culture Reproductive technology Biology Virus Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Reproductive Medicine Immunology Genetics Animal Science and Zoology Folliculogenesis Molecular Biology Spermatogenesis Gametogenesis 030304 developmental biology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 27:170 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv27n1ab158 |
| Popis: | BoHV4 is a double-stranded DNA virus which has been associated to endometritis, metritis, and abortions in dairy cow. The objective of this study was to characterise its cytopathic effects on bovine endometrial epithelial cells (bEEC). Bovine uteri were collected from slaughter house and bEEC separated and cultivated as previously described (Guo et al. 2014 Reprod. Fertil. Dev. 26, 165–166). In Experiment 1 (Exp 1), bEEC (passage 5) from 3 cows were cultivated for 6 days without virus or following exposure to serial dilutions (10–4, 10–3, 10–2) of virus. Living cells were counted for each group at start of the experiment and by Day 6. Proliferation or inhibition of proliferation was calculated by (Number of cells Day 6 – Number of cells Day 0)/Number of cells Day 0. In Experiment 2 (Exp 2) cells were challenged with a single dosage of virus (MOI 0.01; 1 virus: 100 cells) and culture performed during 1, 2, 3, 4, 5, 6, or 7 days. Cells were counted at Day 0 and each day, proliferation of cells was calculated as (number of cells by Day X – number of cells Day 0)/number of cells Day 0. The effects of the dilution of virus, cow and their interaction (Exp 1) or effects of time, cow, viral exposure, and second-order interactions (Exp 2) on cow cell proliferation were analysed by ANOVA (SAS 9.2, proc GLM; SAS Institute, Inc., Cary, NC, USA). In Exp 1, the amount of living cells by Day 6 was very significantly increased in controls when compared to Day 0 (+172.6 ± 24%; P |
| Databáze: | OpenAIRE |
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