Application of protic ionic liquids in the microwave-assisted extraction of phycobiliproteins from Arthrospira platensis with antioxidant activity
| Autor: | Renata Débora Pinto Rodrigues, Rílvia Saraiva de Santiago-Aguiar, Maria Valderez Ponte Rocha, Thalita Adrielly Viana Carlos, Amanda de Sousa e Silva, Ana Karine Pessoa Bastos |
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| Rok vydání: | 2020 |
| Předmět: |
Allophycocyanin
Chromatography Phycobiliprotein Extraction (chemistry) Filtration and Separation 02 engineering and technology 021001 nanoscience & nanotechnology Chloride Analytical Chemistry Solvent chemistry.chemical_compound 020401 chemical engineering chemistry Ionic liquid Phycocyanin medicine Formate 0204 chemical engineering 0210 nano-technology medicine.drug |
| Zdroj: | Separation and Purification Technology. 252:117448 |
| ISSN: | 1383-5866 |
| DOI: | 10.1016/j.seppur.2020.117448 |
| Popis: | Phycobiliproteins are potential molecules for use in various applications. Although phycobiliproteins extraction classical methods are reproducible and show good yields, they are generally time-consuming and require the use of high energy consumption and/or large volumes of solvent. Therefore, it is necessary the development of new eco-friendlier process by extraction techniques, permitting the transfer of the results obtained in laboratory scale for industrial. Then, microwave-assisted extraction using protic ionic liquid as solvent was proposed and optimized to extract phycobiliproteins from Arthrospira platensis in an efficient manner. The protic ionic liquids (PILs): 2-hydroxyethylammonium acetate (2-HEAA), 2-hydroxyethylammonium formate (2-HEAF), their equimolar mixture (2-HEAA + 2-HEAF), and the commercial ionic liquid 1-butyl-3-methylimidazolium chloride [Bmim]Cl were used as solvents, and sodium phosphate buffer as a control and the conditions of extraction were evaluated. The irradiation power was the most significant variable for the extraction process, and it showed a negative effect. The process conducted at 62 W and pH 7.0, using 2-HEAA + 2-HEAF and a solvent: biomass ratio of 10 mL∙g−1, showed the highest phycobiliproteins concentrations. The purity obtained for phycocyanin, allophycocyanin and phycoerythrin were 1.22, 1.03, and 0.71, respectively. The mixture of the PILs (2-HEAA + 2-HEAF) can be reused for three cycles for extraction of allophycocyanin. The extraction of phycobiliproteins using microwave and ionic liquids was efficient and the degree of purity and antioxidant activity achieved allows the application of these pigments in food and cosmetics. |
| Databáze: | OpenAIRE |
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