A new medical device for in vivo capturing of circulating tumor cells in breast cancer (BC) patients

Autor:	Stefanie Herold, Arndt Schmitz, Klaus Luecke, Dawid Murawa, Phillip Kim, Piotr Nowaczyk, Nils G. Morgenthaler, Paweł Murawa, Maciej Zabel, Thomas Krahn
Rok vydání:	2012
Předmět:	Oncology Cancer Research medicine.medical_specialty Medical device business.industry medicine.disease Treatment efficacy Circulating tumor cell Breast cancer In vivo Internal medicine Medicine Biomarker (medicine) business
Zdroj:	Journal of Clinical Oncology. 30:10537-10537
ISSN:	1527-7755 0732-183X
DOI:	10.1200/jco.2012.30.15_suppl.10537
Popis:	10537 Background: In BC, the number of circulating tumor cells (CTCs) is discussed as a prognostic and stratification biomarker, and could also reflect treatment efficacy. Currently, CTCs are isolated ex vivo from a small volume of blood. Results from a larger volume of blood are scarce. The aim of the study was to assess a functionalized and structured medical wire (FSMW) for in vivo capturing of CTCs directly from the blood stream of BC patients. Methods: The device was inserted in a cubital vein through a standard cannula for thirty minutes. The interaction of target CTCs with the FSMW was mediated by antibodies directed against the epithelial cell adhesion molecule (EpCAM). To confirm binding of CTCs to the wire, the immunohistochemical positive staining against EpCAM as well as negative staining for CD45 was performed. There were 54 applications of the wire in 42 stage I-IV BC patients (12 double applications). Enumeration data from 37 BC patients with 49 applications (5 failed subsequent analyses) were assessed. CTC counts on 23 devices were directly compared to counts by CellSearch. Results: The device was well tolerated in all 54 applications without side effects. We obtained in vivo isolation of CTCs in 44 of 49 applications to BC patients (89.7 %). The sensitivity was similar for early and late stage BC patients. The median (range) of isolated EpCAM-positive CTCs was 5 (0-515). The CellSearch method reached a sensitivity of 18.5%. In all paired samples the number of CTCs detected with the FSMW was higher or equal to CellSearch, regardless of the disease stage. Linear regression of the data of the double application of the FSMW showed a very good concordance (r2 = 0.97, p
Databáze:	OpenAIRE
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