| Autor: |
Shakirah Razali, Al’aina Firus Khan, Alfi Khatib, Qamar Uddin Ahmed, Habibah Hassan, Suganya Murugesu, Tara Jalal, Muhammad Ichwan, Jinap Selamat, Shaden Khalifa, Mohamed Abdel-Daim, Hesham El-Seedi, Ridhwan Abdul Wahab |
| Rok vydání: |
2021 |
| Popis: |
Neolamarckia cadamba (NC) leaf is traditionally used for the treatment of breast cancer, however this claim is unverified. This study aimed to evaluate the anti-cancer activities of NC leaf ethanol extract on breast cancer cell line (MCF-7 cells) using in vitro cell viability, cytotoxicity and gene expression assays followed by gas chromatography analysis. Results revealed inhibition concentration (IC50) against MCF-7 at 0.2 mg/mL. The extract exerted a dose and time dependent inhibitory effect against MCF-7 cells. The cell cycle assay showed that the extract arrested MCF-7 cells in G0/G1 phase, and apoptosis were observed after 72 hours by Annexin-V assay. The gene expression assay revealed that the cell cycle arrest was associated with the down-regulation of CDK2 and subsequent up-regulation of p21 and cyclin E. The extract induced apoptosis via mediation of the mitochondrial cell death pathways. Chromatography analysis revealed the contribution of d-pinitol and myo-inositol to the activity observed as the two major bioactive compounds. Overall, the study demonstrated that NC exerts anti-cancer effect on MCF-7 human breast cancer cells through induction of apoptosis and cell cycle arrest thus justifying its traditional use for breast cancer treatment in Malaysia. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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