Hepcidin messenger RNA expression in human lymphocytes
| Autor: | Helena Carmo, J. E. B. P. Pinto, Maria de Sousa, Vera Dias, Heinz Zoller, Félix Carvalho, Graça Porto |
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| Rok vydání: | 2010 |
| Předmět: |
inorganic chemicals
congenital hereditary and neonatal diseases and abnormalities Messenger RNA Innate immune system biology Immunology Ferroportin nutritional and metabolic diseases Lymphocyte proliferation Molecular biology Peripheral blood mononuclear cell Hepcidin hemic and lymphatic diseases biology.protein Immunology and Allergy Gene silencing Intracellular |
| Zdroj: | Immunology. 130:217-230 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1111/j.1365-2567.2009.03226.x |
| Popis: | Hepcidin regulates intracellular iron levels by interacting with and promoting the degradation of ferroportin, a membrane protein and the only known cellular iron exporter. Studies of hepcidin expression and regulation have focused on its effects in innate immunity and as a regulator of systemic iron metabolism. In the present study we characterized the expression of hepcidin messenger RNA (mRNA) in human peripheral blood mononuclear cells (PBMCs) with a focus on peripheral blood lymphocytes (PBLs). We found that (1) all human PBMCs analyzed express basal hepcidin mRNA levels; (2) hepcidin mRNA expression increases after T-lymphocyte activation; (3) expression by PBLs increases in response to challenge by holotransferrin (Fe-TF) and by ferric citrate in vitro; (4) the Fe-TF-mediated up-regulation of hepcidin decreases ferroportin expression at the cytoplasmic membrane of PBLs; and (5) silencing of tumour necrosis factor-alpha (TNF-alpha) abrogates the effect of Fe-TF. In summary, we show that hepcidin expression determines intracellular iron levels by regulating the expression of ferroportin, as described in other cells, and that inappropriately low expression of hepcidin impairs normal lymphocyte proliferation. The results establish hepcidin as a new player in lymphocyte biology. |
| Databáze: | OpenAIRE |
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