Thermo-responsive allele of sucrose synthase 3 (Sus3) provides high-temperature tolerance during the ripening stage in rice (Oryza sativa L.)
| Autor: | Kohei Yamaguchi, Genki Chaya, Yukimoto Iwasaki, Kotaro Miura, Kazumasa Murata, Takeshi Ebitani, Kana Takehara, Hitoshi Ogiwara, Takuya Yamaguchi, Shintaro Kido |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Oryza sativa biology food and beverages Ripening Locus (genetics) Plant Science Quantitative trait locus biology.organism_classification 01 natural sciences Japonica 03 medical and health sciences Horticulture 030104 developmental biology Genetics biology.protein Sucrose synthase Brown rice Allele Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Breeding Science. 68:336-342 |
| ISSN: | 1347-3735 1344-7610 |
| DOI: | 10.1270/jsbbs.18007 |
| Popis: | High-temperature stress during the ripening stage leads to quality deterioration due to an increase in chalky grains in brown rice (Oryza sativa L.). In a previous study, we identified a QTL for Appearance quality of brown rice 1 (Apq1) using chromosome segment substitution lines of the indica cultivar 'Habataki' in the japonica cultivar 'Koshihikari' background and narrowed down the locus to a 48-kb region on chromosome 7. To verify the function and mechanisms of this QTL in grain appearance, in this study, we fine-mapped the gene and conducted high-temperature tolerance tests. As a result of the genetic mapping, we narrowed down the candidate region of Apq1 to a 19.4-kb region including three predicted genes. Among these, the temporal expression pattern of sucrose synthase 3 (Sus3) corresponded well with the high temperature-sensitive period during ripening, and expression of the 'Habataki' allele of Sus3 was increased under high-temperature condition. In addition, we transformed the 'Habataki' Sus3 gene into 'Nipponbare', and the transformants obtained high-temperature tolerance. Therefore, we conclude that the causal gene underlying the QTL Apq1 is the thermo-responsive Sus3 allele, and the increase in Sus3 expression under high-temperature condition during ripening leads to high-temperature tolerance in rice. |
| Databáze: | OpenAIRE |
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