Fructooligosaccharides synthesized by fructosyltransferase from an indigenous coprophilous Aspergillus niger strain XOBP48 exhibits antioxidant activity
| Autor: | Samson Mukaratirwa, Ajit Kumar, Jeff Ojwach, Taurai Mutanda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vitamin
0303 health sciences Sucrose Antioxidant biology 030309 nutrition & dietetics DPPH medicine.medical_treatment Organic Chemistry Aspergillus niger 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Biochemistry Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology chemistry Biotransformation medicine Ferric Food science Food Science medicine.drug |
| Zdroj: | Bioactive Carbohydrates and Dietary Fibre. 24:100238 |
| ISSN: | 2212-6198 |
| Popis: | Fructooligosaccharides (FOS) produced via sucrose biotransformation by partially purified Fructosyltransferase (Ftase) from an indigenous coprophilous Aspergillus niger strain XOBP48 (AnXOBP48) are reported to have antioxidant and nutraceutical properties in the present study. The Ftase activity on sucrose yielded FOS identified as monomeric glucose, 1- kestose (GF2), and 1,1– kestotetraose (GF3) which were further purified and quantified using HPLC-RI system. The antioxidant activities of purified FOS at concentrations of 1, 1.5, 3, 6, 12, 24 and 48 μg/ml were determined by applying three experimental models and comparing their properties with FOS standards and vitamin C. The free radical scavenging activity measured by 1,1 - diphenyl-2-picryl hydroxyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay and nitric oxide (NO) radical inhibition assay, yielded IC50 values of 2.6 μg/ml, 3.9 μg/ml and 3.4 μg/ml, 3.8 μg/ml and 0.69 μg/ml, 0.74 for purified GF2 and GF3, respectively. The free radical scavenging and inhibition activities showed a concentration dependent antioxidant activity of purified FOS with no significant differences as compared to the standards p |
| Databáze: | OpenAIRE |
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